Capillary electrophoresis with field-enhanced stacking for rapid and sensitive determination of strychnine and brucine

Jiyou Zhang; Shufang Wang; Xingguo Chen; Zhide Hu; Xiao Ma

doi:10.1007/s00216-003-1852-y

Capillary electrophoresis with field-enhanced stacking for rapid and sensitive determination of strychnine and brucine

Anal Bioanal Chem. 2003 May;376(2):210-3. doi: 10.1007/s00216-003-1852-y. Epub 2003 Apr 12.

Authors

Jiyou Zhang¹, Shufang Wang, Xingguo Chen, Zhide Hu, Xiao Ma

Affiliation

¹ Department of Chemistry, Lanzhou University, 730000 Lanzhou, PR China.

PMID: 12692702
DOI: 10.1007/s00216-003-1852-y

Abstract

A new capillary electrophoresis procedure with field-enhanced stacking concentration for the analysis of strychnine and brucine is established. After optimization of the separation and concentration conditions, the two alkaloids can be separated within 5 min and quantified with high sensitivity (The detection limits were 1.0 ng mL(-1) for strychnine and 1.4 ng mL(-1) for brucine). The method was useful for qualitative and quantitative analysis of strychnine and brucine in Strychnos nux-vomica L with recovery of 105.1% for strychnine and 98.4% for brucine.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Drugs, Chinese Herbal / chemistry
Electrophoresis, Capillary / methods*
Hydrogen-Ion Concentration
Magnoliopsida / chemistry
Sensitivity and Specificity
Strychnine / analogs & derivatives*
Strychnine / analysis*
Strychnine / isolation & purification
Strychnos nux-vomica

Substances

Drugs, Chinese Herbal
brucine
Strychnine