Abstract
To investigate whether bee venom (BV) induces apoptosis, the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, terminal deoxynucleotidyl transferase- mediated dUTP nick end-labeling assay, 4,6-diamidino-2-phenylindole staining, flow cytometric analysis, and DNA fragmentation assay were performed on NCI-H1299 lung cancer cells treated with BV. Through morphological and biochemical analyses, it was demonstrated that NCI-H1299 cells treated with BV exhibit several features of apoptosis. In addition, reverse transcription-polymerase chain reaction and prostaglandin E(2) (PGE(2)) immunoassay were performed to verify whether BV possesses an inhibitory effect on the expression of cyclooxygenase (COX) and PGE(2 )synthesis. Expression of COX-2 mRNA and synthesis of PGE(2) were inhibited by BV. These results suggest the possibility that BV may exert an anti-tumor effect on human lung cancer.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Apoptosis / drug effects*
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Bee Venoms / pharmacology*
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Caspases / metabolism
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Cell Cycle / drug effects
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Cell Line
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Cell Survival / drug effects
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Cyclooxygenase 1
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Cyclooxygenase 2
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Cyclooxygenase 2 Inhibitors
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Cyclooxygenase Inhibitors*
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DNA Fragmentation / drug effects
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Dinoprostone / metabolism
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Flow Cytometry
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Fluorescent Dyes
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Gene Expression Regulation, Enzymologic / drug effects*
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Genes, bcl-2 / drug effects
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Humans
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In Situ Nick-End Labeling
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Indicators and Reagents
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Indoles
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Isoenzymes / biosynthesis*
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Isoenzymes / genetics
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Lung Neoplasms / enzymology*
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Membrane Proteins
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Prostaglandin-Endoperoxide Synthases / biosynthesis*
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Prostaglandin-Endoperoxide Synthases / genetics
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Proto-Oncogene Proteins / biosynthesis
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Proto-Oncogene Proteins c-bcl-2*
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RNA, Messenger / biosynthesis*
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RNA, Messenger / genetics
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RNA, Messenger / isolation & purification
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Reverse Transcriptase Polymerase Chain Reaction
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Tetrazolium Salts
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Thiazoles
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bcl-2-Associated X Protein
Substances
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Bee Venoms
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Cyclooxygenase 2 Inhibitors
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Cyclooxygenase Inhibitors
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Fluorescent Dyes
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Indicators and Reagents
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Indoles
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Isoenzymes
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Membrane Proteins
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Proto-Oncogene Proteins
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Proto-Oncogene Proteins c-bcl-2
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RNA, Messenger
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Tetrazolium Salts
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Thiazoles
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bcl-2-Associated X Protein
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DAPI
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Cyclooxygenase 1
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Cyclooxygenase 2
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PTGS1 protein, human
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PTGS2 protein, human
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Prostaglandin-Endoperoxide Synthases
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Caspases
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thiazolyl blue
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Dinoprostone