Calbindin D28K interacts with Ran-binding protein M: identification of interacting domains by NMR spectroscopy

Biochem Biophys Res Commun. 2003 Apr 18;303(4):1186-92. doi: 10.1016/s0006-291x(03)00499-6.

Abstract

Calbindin D(28K) is an EF-hand containing protein that plays a vital role in neurological function. We now show that calcium-loaded calbindin D(28K) interacts with Ran-binding protein M, a protein known to play a role in microtubule function. Using NMR methods, we show that a peptide, LASIKNR, derived from Ran-binding protein M, interacts with several regions of the calcium-loaded protein including the amino terminus and two other regions that exhibit conformational exchange on the NMR timescale. We suggest that the interaction between calbindin D(28K) and Ran-binding protein M may be important in calbindin D(28K) function.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adaptor Proteins, Signal Transducing
  • Amino Acid Sequence
  • Binding Sites
  • Calbindins
  • Cytoskeletal Proteins
  • Magnetic Resonance Spectroscopy
  • Molecular Sequence Data
  • Nuclear Proteins / chemistry
  • Nuclear Proteins / metabolism*
  • Precipitin Tests
  • Protein Structure, Tertiary
  • S100 Calcium Binding Protein G / chemistry*
  • S100 Calcium Binding Protein G / metabolism*
  • ran GTP-Binding Protein / chemistry
  • ran GTP-Binding Protein / metabolism*

Substances

  • Adaptor Proteins, Signal Transducing
  • Calbindins
  • Cytoskeletal Proteins
  • Nuclear Proteins
  • Ran binding protein 9
  • S100 Calcium Binding Protein G
  • ran GTP-Binding Protein