An HPLC method was established for the determination of hesperidin and naringin in the Fructus Aurantii Immaturus and Fructus Aurantii from different places. Hesperidin and narigin were separated on a Hypersil ODS1 column with acetonitrile-0.5% acetic acid (volume ratio of 22:78) as the mobile phase and detected at 283 nm. The flow rate was 1.0 mL/min. The column temperature was set at 35 degrees C. The calibration curves were linear in the ranges of 0.060 g/L-0.604 g/L and 0.0125 g/L-0.125 g/L for naringin and hesperidin respectively. The average recoveries were 97.1% for naringin and 95.3% for hesperidin. The method is simple, rapid, accurate and reliable.