New PCR test that recognizes all human prototypes of enterovirus: application for clinical diagnosis

J Clin Microbiol. 2003 Apr;41(4):1750-2. doi: 10.1128/JCM.41.4.1750-1752.2003.

Abstract

We describe a new PCR test (Penter RT-PCR) that recognizes all 64 prototypes of enterovirus. Sixty clinical samples were analyzed in parallel with this Penter RT-PCR and previously described PCR tests: 34 and 32 samples tested positive, respectively. This assay is suitable for use in clinical diagnosis, and its ability to amplify all known serotypes makes it more useful than other consensus PCR tests.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Enterovirus / classification*
  • Enterovirus / genetics*
  • Enterovirus Infections / diagnosis*
  • Enterovirus Infections / virology
  • Humans
  • Reverse Transcriptase Polymerase Chain Reaction / methods*
  • Serotyping