Use of monoclonal antibodies to lipopolysaccharide for antigenic analysis of Coxiella burnetii

Akitoyo Hotta; Midori Kawamura; Ho To; Masako Andoh; Tsuyoshi Yamaguchi; Hideto Fukushi; Ken-Ichi Amano; Katsuya Hirai

doi:10.1128/JCM.41.4.1747-1749.2003

Use of monoclonal antibodies to lipopolysaccharide for antigenic analysis of Coxiella burnetii

J Clin Microbiol. 2003 Apr;41(4):1747-9. doi: 10.1128/JCM.41.4.1747-1749.2003.

Authors

Akitoyo Hotta¹, Midori Kawamura, Ho To, Masako Andoh, Tsuyoshi Yamaguchi, Hideto Fukushi, Ken-Ichi Amano, Katsuya Hirai

Affiliation

¹ Department of Veterinary Microbiology, Faculty of Agriculture, Gifu University, 1-1 Yanagido, Gifu, Gifu 501-1193, Japan.

Abstract

Antigenic differences among Coxiella burnetii strains were analyzed. The monoclonal antibodies against the lipopolysaccharide outer core did not react with the strains containing a QpRS plasmid or with plasmidless strains, whereas they reacted with strains containing a QpH1 or QpDV plasmid. C. burnetii isolates could be divided into two groups immunologically.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Antibodies, Bacterial / immunology*
Antibodies, Monoclonal / immunology*
Antigens, Bacterial / analysis*
Blotting, Western
Cattle
Coxiella burnetii / classification*
Coxiella burnetii / immunology
Humans
Lipopolysaccharides / immunology*
Mice
Plasmids
Q Fever / diagnosis
Serotyping

Substances

Antibodies, Bacterial
Antibodies, Monoclonal
Antigens, Bacterial
Lipopolysaccharides