Screening method for Salmonella enterica serovar Typhi and serovar Paratyphi A with reduced susceptibility to fluoroquinolones by PCR-restriction fragment length polymorphism

Microbiol Immunol. 2003;47(2):161-5. doi: 10.1111/j.1348-0421.2003.tb02800.x.

Abstract

Salmonella enterica serovar Typhi and serovar Paratyphi A with reduced susceptibility to fluoroquinolones (MICs of ciprofloxacin, 0.25 to 2 microg/ml) have a mutation at codon either Ser-83 or Asp-87 of gyrA gene. A screening method by PCR-restriction fragment length polymorphism (PCR-RFLP) was designed to screen the mutations at codon Ser-83 and Asp-87 of the gyrA gene of S. enterica serovar Typhi and serovar Paratyphi A clinical isolates. This method successfully screened the gyrA mutations of S. enterica serovar Typhi and serovar Paratyphi A with reduced susceptibility to fluoroquinolones.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anti-Infective Agents / pharmacology*
  • DNA Gyrase / genetics
  • DNA Gyrase / metabolism
  • Drug Resistance, Microbial / genetics*
  • Fluoroquinolones
  • Humans
  • Microbial Sensitivity Tests
  • Mutation
  • Paratyphoid Fever / microbiology
  • Polymerase Chain Reaction / methods*
  • Polymorphism, Restriction Fragment Length*
  • Salmonella paratyphi A / drug effects*
  • Salmonella paratyphi A / enzymology
  • Salmonella paratyphi A / genetics
  • Salmonella typhi / drug effects*
  • Salmonella typhi / enzymology
  • Salmonella typhi / genetics
  • Typhoid Fever / microbiology

Substances

  • Anti-Infective Agents
  • Fluoroquinolones
  • DNA Gyrase