Abstract
Salmonella enterica serovar Typhi and serovar Paratyphi A with reduced susceptibility to fluoroquinolones (MICs of ciprofloxacin, 0.25 to 2 microg/ml) have a mutation at codon either Ser-83 or Asp-87 of gyrA gene. A screening method by PCR-restriction fragment length polymorphism (PCR-RFLP) was designed to screen the mutations at codon Ser-83 and Asp-87 of the gyrA gene of S. enterica serovar Typhi and serovar Paratyphi A clinical isolates. This method successfully screened the gyrA mutations of S. enterica serovar Typhi and serovar Paratyphi A with reduced susceptibility to fluoroquinolones.
Publication types
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Evaluation Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Anti-Infective Agents / pharmacology*
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DNA Gyrase / genetics
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DNA Gyrase / metabolism
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Drug Resistance, Microbial / genetics*
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Fluoroquinolones
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Humans
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Microbial Sensitivity Tests
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Mutation
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Paratyphoid Fever / microbiology
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Polymerase Chain Reaction / methods*
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Polymorphism, Restriction Fragment Length*
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Salmonella paratyphi A / drug effects*
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Salmonella paratyphi A / enzymology
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Salmonella paratyphi A / genetics
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Salmonella typhi / drug effects*
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Salmonella typhi / enzymology
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Salmonella typhi / genetics
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Typhoid Fever / microbiology
Substances
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Anti-Infective Agents
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Fluoroquinolones
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DNA Gyrase