Abstract
c-Jun is a member of the AP-1 family of transcription factors regulating expression of specific target genes in a variety of cellular processes including proliferation, stress response, and tumorigenicity. In the present study we have analyzed the mechanism of c-Jun function as a transactivator with respect to members of the basal transcription machinery, TATA-binding protein-associated factors (TAFs). We show that one member of the family, human TAF7 (formerly TAFII55), physically interacts with c-Jun through two independent interaction domains, within the N- and C-terminal part of c-Jun. Interaction in vitro correlates with enhanced transactivation function of c-Jun in HEK293 and COS cells in the presence of increasing amounts of TAF7. TAF7 interacts preferentially with DNA-bound phosphorylated c-Jun, suggesting that TAF7 represents a novel c-Jun co-activator mediating activation of AP-1 target genes in response to extracellular signals.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Blotting, Western
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COS Cells
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Cell Line
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Glutathione Transferase / metabolism
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Humans
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JNK Mitogen-Activated Protein Kinases
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Luciferases / metabolism
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Mitogen-Activated Protein Kinases / metabolism*
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Oligonucleotides / chemistry
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Phosphorylation
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Plasmids / metabolism
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Precipitin Tests
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Protein Binding
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Protein Structure, Tertiary
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Recombinant Proteins / metabolism
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TATA-Binding Protein Associated Factors / metabolism*
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TATA-Binding Protein Associated Factors / physiology*
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Transcription Factor AP-1 / metabolism
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Transcription Factor TFIID / metabolism*
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Transcription Factor TFIID / physiology*
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Transcription, Genetic*
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Transcriptional Activation
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Transfection
Substances
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Oligonucleotides
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Recombinant Proteins
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TAF7 protein, human
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TATA-Binding Protein Associated Factors
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Transcription Factor AP-1
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Transcription Factor TFIID
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Luciferases
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Glutathione Transferase
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JNK Mitogen-Activated Protein Kinases
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Mitogen-Activated Protein Kinases