Abstract
A new and three known pyridoacridine alkaloids were isolated from the Indonesian marine sponge Biemna fortis as neuronal differentiation inducers against a murine neuroblastoma cell line, Neuro 2A. The chemical structure of the new compound, labuanine A (1), was determined by spectroscopic study and chemical conversion. These pyridoacridine alkaloids induced multipolar neuritogenesis in more than 50% of cells at 0.03-3 micro M concentration. Compound 3, which showed the strongest neuritogenic activity among them, also induced increase of acetylcholinesterase, a neuronal marker in Neuro 2A and arrested cell cycle at the G2/M phase.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Acridines / chemistry
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Acridines / isolation & purification
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Acridines / pharmacology*
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Alkaloids / chemistry
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Alkaloids / isolation & purification
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Alkaloids / pharmacology*
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Animals
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Cell Differentiation / drug effects*
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Cell Differentiation / physiology
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Cell Line, Tumor / cytology
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Cell Line, Tumor / drug effects
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Cell Transformation, Neoplastic / drug effects
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Cell Transformation, Neoplastic / pathology
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Mice
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Neuroblastoma
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Neurons / cytology
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Neurons / drug effects*
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Phenanthrolines / chemistry
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Phenanthrolines / isolation & purification
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Phenanthrolines / pharmacology*
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Porifera*
Substances
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Acridines
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Alkaloids
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Phenanthrolines
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pyridoacridine