Modulation of BmK AS, a scorpion neurotoxic polypeptide, on voltage-gated Na+ channels in B104 neuronal cell line

Neurosci Lett. 2003 Apr 10;340(2):123-6. doi: 10.1016/s0304-3940(03)00094-6.

Abstract

The modulation of BmK AS, a neurotoxic polypeptide from scorpion Buthus martensi Karsch, on the voltage-gated Na(+) channels has been investigated in the B104 neuroblastoma cell line by whole-cell patch clamping. It was found that the whole Na(+) currents and tetrodotoxin-resistant Na(+) current were depressed in a biphasic manner and both steady-state activation and inactivation curves were shifted toward the hyperpolarizing direction in the presence of BmK AS. The results suggested that BmK AS could inhibit both tetrodotoxin-sensitive and -resistant Na(+) currents via modulating the gating mechanism of the voltage-gated Na(+) channels in B104 cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Dose-Response Relationship, Drug
  • Neurons / drug effects*
  • Neurons / physiology
  • Peptides / toxicity*
  • Rats
  • Scorpion Venoms / toxicity*
  • Sodium Channels / physiology*
  • Tetrodotoxin / pharmacology
  • Tumor Cells, Cultured

Substances

  • BmK AS polypeptide
  • Peptides
  • Scorpion Venoms
  • Sodium Channels
  • Tetrodotoxin