Abstract
Set1, the yeast histone H3-lysine 4 (H3-K4) methylase, is recruited by the Pol II elongation machinery to a highly localized domain at the 5' portion of active mRNA coding regions. Set1 association depends upon the TFIIH-associated kinase that phosphorylates the Pol II C-terminal domain (CTD) and mediates the transition between initiation and elongation, and Set1 interacts with the form of Pol II whose CTD is phosphorylated at serine 5 but not serine 2. The Rtf1 and Paf1 components of the Pol II-associated Paf1 complex are also important for Set1 recruitment. Although the level of dimethylated H3-K4 is fairly uniform throughout the genome, the pattern of trimethylated H3-K4 strongly correlates with Set1 occupancy. Hypermethylated H3-K4 within the mRNA coding region persists for considerable time after transcriptional inactivation and Set1 dissociation from the chromatin, indicating that H3-K4 hypermethylation provides a molecular memory of recent transcriptional activity.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Blotting, Western
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DNA Methylation
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DNA Polymerase II / metabolism*
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DNA-Binding Proteins / chemistry*
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DNA-Binding Proteins / metabolism*
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Genome, Fungal
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Histone-Lysine N-Methyltransferase
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Histones / metabolism
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Ligases / metabolism
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Methylation
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Models, Genetic
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Nuclear Proteins / metabolism
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Open Reading Frames
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Phosphorylation
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Precipitin Tests
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Protein Structure, Tertiary
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RNA, Messenger / metabolism
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Saccharomyces cerevisiae / metabolism
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Saccharomyces cerevisiae Proteins / chemistry*
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Saccharomyces cerevisiae Proteins / metabolism*
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Serine / metabolism
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TATA-Box Binding Protein / metabolism
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Transcription Factors / chemistry*
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Transcription Factors / metabolism*
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Transcription, Genetic*
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Ubiquitin-Conjugating Enzymes
Substances
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DNA-Binding Proteins
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Histones
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Nuclear Proteins
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PAF1 protein, S cerevisiae
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RNA, Messenger
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RTF1 protein, S cerevisiae
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Saccharomyces cerevisiae Proteins
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TATA-Box Binding Protein
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Transcription Factors
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Serine
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Histone-Lysine N-Methyltransferase
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SET1 protein, S cerevisiae
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Ubiquitin-Conjugating Enzymes
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DNA Polymerase II
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Ligases