Objective: To explore the effects of swine bone marrow derived mesenchymal stem cells (MSC) on the repair of skin wound combined local irradiation injury and theirs mechanism.
Methods: MSC were isolated from the adult swine bone marrow and autologous MSC were directly transplanted into the local site full thickness skin incision round wounds combined with 20 Gy irradiation. By means of laser confocal microscopy, quantitative collagen measurement, ELSIA, fibroblasts scrap test, RT-PCR technology and so on in order to observe the effects of MSC promote the repair of wound and theirs mechanism.
Results: Transplanted autologous MSC into local skin wound combined with 20 Gy irradiation could accelerate the speed of wound healing, the healing time moved up 3 - 4 days. The granule tissues were abundant in the wounds and the content of collagen was increased in MSC groups. On one hand, MSC attracted the inflammatory cells and repaired cells to immigrate into wounds by themselves or secretion of IL-6, IL-8 and G-CSF and thus initiated the repairing processes. On the other hand, the wound microenviroment induced the proliferation and changes of I collagen mRNA expression of MSC showed that MSC could secrete extracellular matrix or differentiate into fibroblast.
Conclusion: Local transplanted MSC promoted the repair of skin wounds combined with irradiation injury. The promotion effects were achieved by the interaction between MSC and wound microenviroment.