Ganglioside binding pattern of CD33-related siglecs

Bioorg Med Chem Lett. 2003 Feb 24;13(4):675-8. doi: 10.1016/s0960-894x(02)00998-8.

Abstract

Our study deals with the interaction of CD33 related-siglecs-5,-7,-8,-9,-10 with gangliosides GT1b, GQ1b, GD3, GM2, GM3 and GD1a. Siglec-5 bound preferentially to GQ1b, but weakly to GT1b, whereas siglec-10 interacted only with GT1b ganglioside. Siglec-7 and siglec-9 displayed binding to gangliosides GD3, GQ1b and GT1b bearing a disialoside motif, though siglec-7 was more potent; besides, siglec-9 interacted also with GM3. Siglec-8 demonstrated low affinity to the gangliosides tested compared with other siglecs. Despite high structural similarity of CD33 related siglecs, they demonstrated different ganglioside selectivity, in particular to the Neu5Acalpha2-8Neu5Ac motif.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antigens, CD / chemistry
  • Antigens, CD / metabolism*
  • Antigens, Differentiation, B-Lymphocyte
  • Antigens, Differentiation, Myelomonocytic / chemistry
  • Antigens, Differentiation, Myelomonocytic / metabolism*
  • Carbohydrate Sequence
  • G(M2) Ganglioside
  • G(M3) Ganglioside
  • Gangliosides / chemistry
  • Gangliosides / metabolism*
  • Humans
  • Immunoenzyme Techniques
  • Lectins
  • Protein Binding
  • Receptors, Cell Surface
  • Sialic Acid Binding Ig-like Lectin 3
  • Sialic Acid Binding Immunoglobulin-like Lectins

Substances

  • Antigens, CD
  • Antigens, Differentiation, B-Lymphocyte
  • Antigens, Differentiation, Myelomonocytic
  • CD33 protein, human
  • G(M3) Ganglioside
  • Gangliosides
  • Lectins
  • Receptors, Cell Surface
  • SIGLEC10 protein, human
  • SIGLEC5 protein, human
  • SIGLEC7 protein, human
  • SIGLEC8 protein, human
  • SIGLEC9 protein, human
  • Sialic Acid Binding Ig-like Lectin 3
  • Sialic Acid Binding Immunoglobulin-like Lectins
  • ganglioside, GD1a
  • G(M2) Ganglioside
  • trisialoganglioside GT1
  • ganglioside, GD3
  • GQ1b ganglioside