Abstract
Hot water epilepsy (HWE) is a benign and rare form of reflex epilepsy that occurs most commonly in humans. Bdm1 is one of the proteins whose mRNA transcript is overexpressed during HWE in a rat model. We show, by sequence analysis and fold recognition methods, that Bdm1 has strong structural similarities to alpha/beta hydrolases like the thioesterases. A three-dimensional model derived by comparative modeling methods allowed the search for catalytic residues using a flexible functional template characteristic of these enzymes. We predict that Bdm1 might be regulated by homocysteine levels by means of direct participation in degradation pathways.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Binding Sites
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Catalytic Domain / genetics
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Epilepsy, Reflex / enzymology
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Epilepsy, Reflex / genetics
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Humans
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Hydrolases / chemistry*
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Hydrolases / genetics
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Hydrolases / metabolism
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Hydrolysis
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Models, Molecular
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Molecular Sequence Data
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Muscle Proteins / chemistry*
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Muscle Proteins / genetics
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Muscle Proteins / metabolism
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Nerve Tissue Proteins / chemistry*
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Nerve Tissue Proteins / genetics
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Nerve Tissue Proteins / metabolism
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Protein Conformation
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Protein Folding*
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Rats
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Sequence Alignment
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Sequence Homology, Amino Acid
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Substrate Specificity
Substances
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Muscle Proteins
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Ndrg4 protein, rat
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Nerve Tissue Proteins
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Hydrolases