Flow cytometric measurements of neutrophil functions: the dependence on the stimulus to cell ratio

FEMS Immunol Med Microbiol. 2003 Mar 20;35(2):147-52. doi: 10.1016/S0928-8244(02)00462-5.

Abstract

Phagocytosis and antimicrobial killing of neutrophils has been quantitatively determined as a function of the stimulus (Candida albicans) to cell ratio R using two donor collectives containing a total of 115 blood samples. Analysis of the collectives in two different laboratories according to the same flow cytometric protocol for simultaneous measurement of neutrophil functions did not produce statistically significant differences. The number of phagocytosing leukocytes as well as that of killed fungi per leukocyte depends strongly on R. While each phagocytosing neutrophil kills one fungus at low values of R, each neutrophil kills on average 2.5 fungi for large R.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Flow Cytometry / methods*
  • Fungi / immunology
  • Humans
  • Middle Aged
  • Neutrophils / immunology*
  • Neutrophils / physiology
  • Phagocytosis*
  • Respiratory Burst