Abstract
The effect of meliacine (MAS) and two fractions MAB 1 and MAB 2 obtained from it on the in vitro production of TNF-alpha of murine macrophages induced by bacterial lipopolysaccharide (LPS) (from Escherichia coli) was tested. Simultaneous administration of the above fractions (ranging from 14 to 56 microg/ml) and LPS (10 microg/ml) to a macrophage culture significantly increased the amount of TNF-alpha released at 24 h of induction in a dose-dependent manner. Meliacine alone, at a concentration of 56 microg/ml, is a weak inducer of TNF-alpha production.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antiviral Agents / administration & dosage
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Antiviral Agents / pharmacology*
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Antiviral Agents / therapeutic use
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Dose-Response Relationship, Drug
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Escherichia coli
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Female
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Lipopolysaccharides
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Macrophages, Peritoneal / drug effects
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Melia azedarach*
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Mice
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Mice, Inbred BALB C
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Peptides / administration & dosage
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Peptides / pharmacology*
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Peptides / therapeutic use
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Phytotherapy*
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Plant Extracts / administration & dosage
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Plant Extracts / pharmacology
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Plant Extracts / therapeutic use
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Plant Leaves
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Plant Proteins*
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Tumor Necrosis Factor-alpha / drug effects*
Substances
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Antiviral Agents
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Lipopolysaccharides
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Peptides
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Plant Extracts
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Plant Proteins
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Tumor Necrosis Factor-alpha
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meliacin