Abstract
The Argentinean legume Caesalpinia paraguariensis Burk. (Fabaceae) was selected for further fractionation work based on the strong antimicrobial activity of its CH2Cl2-MeOH (1:1 v/v) extract against a host of clinically significant microorganisms, including antibiotic resistant strains. 1D and 2D NMR enabled the identification of the novel benzoxecin derivative caesalpinol along with the known compounds bilobetin, stigma-5-en-3-O-beta-6'-stearoylglucopyranoside, stigma-5-en-3-beta-6'-palmitoylglucopyranoside, stigma-5-en-3-beta-glucopyranoside, oleanolic acid, 3-O-(E)-hydroxycinnamoyl oleanolic acid, betulinic acid, 3-O-(E)-hydroxycinnamoyl betulinic acid, and lupeol from the active fractions. Oleanolic acid was found active against Bacillus subtilis and both methicillin-sensitive and -resistant Staphylococcus aureus with MICs of 8 (17.5 microM), 8 and 64 (140 microM) microg/ml, respectively. The rest of the compounds, however, did not show activity.
Publication types
-
Comparative Study
-
Research Support, U.S. Gov't, Non-P.H.S.
-
Research Support, U.S. Gov't, P.H.S.
MeSH terms
-
Anti-Bacterial Agents
-
Anti-Infective Agents / chemistry*
-
Anti-Infective Agents / isolation & purification
-
Anti-Infective Agents / pharmacology
-
Bacillus subtilis / drug effects
-
Bacteria / drug effects*
-
Betulinic Acid
-
Caesalpinia / chemistry*
-
Candida albicans / drug effects
-
Escherichia coli / drug effects
-
Flavonoids / chemistry
-
Flavonoids / isolation & purification
-
Flavonoids / pharmacology
-
Magnetic Resonance Spectroscopy
-
Methicillin Resistance
-
Microbial Sensitivity Tests
-
Penicillin G / pharmacology
-
Pentacyclic Triterpenes
-
Plant Extracts / chemistry*
-
Plant Extracts / isolation & purification
-
Plant Extracts / pharmacology
-
Staphylococcus aureus / classification
-
Staphylococcus aureus / drug effects
-
Triterpenes / chemistry
-
Triterpenes / isolation & purification
-
Triterpenes / pharmacology
Substances
-
Anti-Bacterial Agents
-
Anti-Infective Agents
-
Flavonoids
-
Pentacyclic Triterpenes
-
Plant Extracts
-
Triterpenes
-
bilobetin
-
Penicillin G
-
Betulinic Acid