Efficient release of overproduced gene products from Escherichia coli BL21(DE3) by lytic infection with newly isolated bacteriophages

Biosci Biotechnol Biochem. 2003 Jan;67(1):198-202. doi: 10.1271/bbb.67.198.

Abstract

Overproduced proteins from Escherichia coli BL21(DE3) were efficiently released with virulent bacteriophages. Leviviridae-like bacteriophages were isolated from soil and used to lyse BL21(DE3) cells transformed with beta-glucosidase, chitinase, or chitosanase genes. This method caused lysis of bacterial cells similar to that by conventional sonication and enabled us to effectively recover and purify the enzymes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Proteins / biosynthesis*
  • Bacterial Proteins / isolation & purification
  • Chitinases / biosynthesis
  • Chitinases / isolation & purification
  • Deoxyribonucleases / chemistry
  • Electrophoresis, Polyacrylamide Gel
  • Escherichia coli / genetics
  • Escherichia coli / metabolism*
  • Escherichia coli / virology
  • Glycoside Hydrolases / biosynthesis
  • Glycoside Hydrolases / isolation & purification
  • Leviviridae / chemistry*
  • Microscopy, Electron
  • RNA, Bacterial / biosynthesis
  • RNA, Bacterial / isolation & purification
  • Ribonucleases / chemistry
  • Soil Microbiology
  • Ultrasonics
  • beta-Glucosidase / biosynthesis
  • beta-Glucosidase / isolation & purification

Substances

  • Bacterial Proteins
  • RNA, Bacterial
  • Deoxyribonucleases
  • Ribonucleases
  • Glycoside Hydrolases
  • chitosanase
  • Chitinases
  • beta-Glucosidase