Abstract
Overproduced proteins from Escherichia coli BL21(DE3) were efficiently released with virulent bacteriophages. Leviviridae-like bacteriophages were isolated from soil and used to lyse BL21(DE3) cells transformed with beta-glucosidase, chitinase, or chitosanase genes. This method caused lysis of bacterial cells similar to that by conventional sonication and enabled us to effectively recover and purify the enzymes.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacterial Proteins / biosynthesis*
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Bacterial Proteins / isolation & purification
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Chitinases / biosynthesis
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Chitinases / isolation & purification
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Deoxyribonucleases / chemistry
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Electrophoresis, Polyacrylamide Gel
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Escherichia coli / genetics
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Escherichia coli / metabolism*
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Escherichia coli / virology
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Glycoside Hydrolases / biosynthesis
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Glycoside Hydrolases / isolation & purification
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Leviviridae / chemistry*
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Microscopy, Electron
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RNA, Bacterial / biosynthesis
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RNA, Bacterial / isolation & purification
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Ribonucleases / chemistry
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Soil Microbiology
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Ultrasonics
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beta-Glucosidase / biosynthesis
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beta-Glucosidase / isolation & purification
Substances
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Bacterial Proteins
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RNA, Bacterial
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Deoxyribonucleases
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Ribonucleases
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Glycoside Hydrolases
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chitosanase
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Chitinases
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beta-Glucosidase