[Cloning of transmembrane domain sequence of EGFR gene]

Wen-Xue Ma; Hai Yu; Ping-Yong Yi; Hong-Chuan Jin; Jie Yan

doi:10.3785/j.issn.1008-9292.2002.04.003

[Cloning of transmembrane domain sequence of EGFR gene]

Zhejiang Da Xue Xue Bao Yi Xue Ban. 2002 Aug;31(4):235-238. doi: 10.3785/j.issn.1008-9292.2002.04.003.

[Article in Chinese]

Authors

Wen-Xue Ma¹, Hai Yu, Ping-Yong Yi, Hong-Chuan Jin, Jie Yan

Affiliation

¹ Cancer Institute, College of Medical Sciences, Zhejiang University, Hangzhou 310009, China.

PMID: 12601900
DOI: 10.3785/j.issn.1008-9292.2002.04.003

Abstract

OBJECTIVE: To clone the transmembrane (TM) domain sequence of EGFR gene and lay a good foundation for constructing the transmembrane expression vector of recombinant superantigens and cytokines. METHODS: A pair of primers special to the sequence encoding TM domain of EGFR gene were synthesized, TM domain fragment was cloned by RT-PCR, and the PCR product of TM domain sequence was ligated with the pGEM-T vector and confirmed by DNA sequencing. RESULTS: TM domain sequence was successfully cloned and verified by DNA sequencing. CONCLUSION: The successful cloning of TM domain sequence provides a basis for the construction of transmembrane fusion protein of Superantigen-TM or Cytokines-TM in cancer biotherapy.