A instrument based on principle of ion chromatography and amperometric detector for quick determination of iodine in urine are introduced in this paper, which covers from signal collection and treatment to data analysis. It presents the principle of hardware and software method to obtain the determination by using computer technology as well as the method of analysis of chromatogram. The instrument possess advantage of single, sensitive and simple. Both of tow stages of calibration curve for iodine 20-150 and 200-1500 micrograms/L respectively showed good linearity with r > 0.999. The detection limit of iodine was 10 micrograms/L(signal to noise ratio 3:1). The relative standard deviation was less than 5% (n = 4), The relative error of measuring urine reference material was less than 3%. Range of iodine in concentration 15 human urine samples by this method was 116.0-1079 micrograms/L.