Abstract
Most of the peptides presented by major histocompatibility complex (MHC) class I molecules require processing by proteasomes. Tripeptidyl peptidase II (TPPII), an aminopeptidase with endoproteolytic activity, may also have a role in antigen processing. Here, we analyzed the processing and presentation of the immunodominant human immunodeficiency virus epitope HIV-Nef(73-82) in human dendritic cells. We found that inhibition of proteasome activity did not impair Nef(73-82) epitope presentation. In contrast, specific inhibition of TPPII led to a reduction of Nef(73-82) epitope presentation. We propose that TPPII can act in combination with or independent of the proteasome system and can generate epitopes that evade generation by the proteasome-system.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Aminopeptidases
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Anti-Bacterial Agents / pharmacology
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Antigen Presentation / immunology
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Cysteine Endopeptidases / metabolism
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Dendritic Cells / immunology
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Dipeptidyl-Peptidases and Tripeptidyl-Peptidases
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Endopeptidases / immunology
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Endopeptidases / metabolism*
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Epitopes / biosynthesis*
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HIV / immunology*
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Histocompatibility Antigens Class I / biosynthesis*
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Histocompatibility Antigens Class I / immunology
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Humans
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Multienzyme Complexes / metabolism
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Oligopeptides / pharmacology
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Proteasome Endopeptidase Complex
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RNA, Small Interfering / metabolism
Substances
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Anti-Bacterial Agents
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Epitopes
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Histocompatibility Antigens Class I
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Multienzyme Complexes
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Oligopeptides
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RNA, Small Interfering
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Endopeptidases
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Aminopeptidases
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Dipeptidyl-Peptidases and Tripeptidyl-Peptidases
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Cysteine Endopeptidases
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Proteasome Endopeptidase Complex
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epoxomicin