Abstract
In prior studies aggregation of the high affinity receptors for IgE, Fc epsilon RI, on a rat mast cell line, RBL-2H3, stimulated transcription of the gene for monocyte chemotactic protein-1 (MCP-1) and secretion of the protein. Unexpectedly, those delayed events appeared much less constrained by kinetic proofreading than had been documented for other receptor-initiated responses. The results of the present experiments are consistent with the proposal that the biosynthesis and secretion of MCP-1 result from a soluble messenger formed in the reaction cascades initiated by the receptor, and that Ca(2+) could serve as that messenger. Interestingly, whereas receptor-mediated signals were required for transcription of the gene for MCP-1 and secretion of the chemokine, such signals were not required for the intervening step of translation of its mRNA.
MeSH terms
-
Animals
-
Calcium / metabolism
-
Calcium Signaling / drug effects
-
Calcium Signaling / immunology
-
Chemokine CCL2 / biosynthesis*
-
Chemokine CCL2 / genetics
-
Chemokine CCL2 / metabolism*
-
Dactinomycin / pharmacology
-
Enzyme-Linked Immunosorbent Assay / methods
-
Intracellular Fluid / drug effects
-
Intracellular Fluid / metabolism
-
Kinetics
-
Ligands
-
Phospholipase C gamma
-
Phosphorylation
-
Protein Biosynthesis / immunology
-
RNA, Messenger / genetics
-
RNA, Messenger / metabolism
-
Rats
-
Receptor Aggregation / immunology
-
Receptors, IgE / metabolism
-
Receptors, IgE / physiology*
-
Transcription, Genetic / drug effects
-
Transcription, Genetic / immunology
-
Tumor Cells, Cultured
-
Type C Phospholipases / physiology
-
Tyrosine / metabolism
Substances
-
Chemokine CCL2
-
Ligands
-
RNA, Messenger
-
Receptors, IgE
-
Dactinomycin
-
Tyrosine
-
Type C Phospholipases
-
Phospholipase C gamma
-
Calcium