[Inhibitory effects of indomethacin and meloxicam on NF-kappa B in mouse peritoneal macrophages]

Yao Xue Xue Bao. 2001 Mar;36(3):161-4.
[Article in Chinese]

Abstract

Aim: To study the inhibitory effects of indomethacin and meloxicam on NF-kappa B from lipopolysaccharide (LPS) stimulated peritoneal macrophages of mice.

Methods: NF-kappa B was measured with the method of electrophoretic mobility shift assay (EMSA).

Results: After induction by LPS at the concentrations of 1 and 3 micrograms.mL-1, the NF-kappa B content of the mouse peritoneal macrophages increased markedly. Indomethacin and meloxicam, at the concentrations of 10(-7)-10(-5) mol.L-1, decreased the activation of NF-kappa B at the concentrations of 1 and 3 micrograms.mL-1 in activated mouse peritoneal macrophages induced with LPS at the concentrations of 1 and 3 micrograms.mL-1.

Conclusion: The inhibitory effects of indomethacin and meloxicam on NF-kappa B activation may be one of their mechanisms of antiinflammatory actions.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Anti-Inflammatory Agents, Non-Steroidal / pharmacology*
  • Electrophoretic Mobility Shift Assay
  • Indomethacin / pharmacology*
  • Lipopolysaccharides
  • Macrophages, Peritoneal / metabolism*
  • Male
  • Meloxicam
  • Mice
  • Mice, Inbred C57BL
  • NF-kappa B / metabolism*
  • Thiazines / pharmacology*
  • Thiazoles / pharmacology*

Substances

  • Anti-Inflammatory Agents, Non-Steroidal
  • Lipopolysaccharides
  • NF-kappa B
  • Thiazines
  • Thiazoles
  • Meloxicam
  • Indomethacin