Aim: To study the inhibitory effects of indomethacin and meloxicam on NF-kappa B from lipopolysaccharide (LPS) stimulated peritoneal macrophages of mice.
Methods: NF-kappa B was measured with the method of electrophoretic mobility shift assay (EMSA).
Results: After induction by LPS at the concentrations of 1 and 3 micrograms.mL-1, the NF-kappa B content of the mouse peritoneal macrophages increased markedly. Indomethacin and meloxicam, at the concentrations of 10(-7)-10(-5) mol.L-1, decreased the activation of NF-kappa B at the concentrations of 1 and 3 micrograms.mL-1 in activated mouse peritoneal macrophages induced with LPS at the concentrations of 1 and 3 micrograms.mL-1.
Conclusion: The inhibitory effects of indomethacin and meloxicam on NF-kappa B activation may be one of their mechanisms of antiinflammatory actions.