Purpose: To establish a new cell line of human retinoblastoma (Rb), frozen and resuscitated to provide a Rb cell reservoir for clinical and experimental research.
Methods: The screening cells were washed and blown directly without centrifugation and then inoculated. Based on the cell-frozen principle, the Rb cell line was collected and established, then frozen in liquid nitrogen and resuscitated.
Results: SO-Rb70 cell line was established successfully. 8 groups of cells were frozen. The resuscitation experiment was carried out for 6 times, 4 times being successful.
Conclusion: The preserved human Rb cells in liquid nitrogen grew normally after resuscitation with the maintenance of previous bioactivities and characteristics. This research helped to provide abundant material for further study of Rb.