[Study on the structural property of polysacchrides 139A--a kind of IL-1R antagonists from microorganism]

J Chen; J B Wu; Y M Liu; G Y Xu

[Study on the structural property of polysacchrides 139A--a kind of IL-1R antagonists from microorganism]

Yao Xue Xue Bao. 2001 Oct;36(10):787-9.

[Article in Chinese]

Authors

J Chen¹, J B Wu, Y M Liu, G Y Xu

Affiliation

¹ Institute of Medical Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China.

PMID: 12579982

Abstract

Aim: To study the structural property of the bioactive component from the fermentation broth of Streptomyces 139 which can inhibit IL-1R.

Methods: Polysaccharide 139A is extracted and purified by means of macroreticular resin Diaion HP-20, cation exchange and DEAE Sephadex A-25 chromatography. The sugar compositions were analyzed with GC, the linkage positions of 139A were determined by methylation and GC-MS.

Results: The bioactive component which can inhibit IL-1R is a kind of polysaccharides.

Conclusion: The components of 139A repeat units are conjectured.

Publication types

English Abstract
Research Support, Non-U.S. Gov't

MeSH terms

Gas Chromatography-Mass Spectrometry
Methylation
Polysaccharides, Bacterial / chemistry
Polysaccharides, Bacterial / isolation & purification*
Polysaccharides, Bacterial / pharmacology
Receptors, Interleukin-1 / antagonists & inhibitors*
Streptomyces / chemistry*

Substances

Polysaccharides, Bacterial
Receptors, Interleukin-1