Objective: To explore the effect of sophora flavescens (SF) combined low molecular weight natural tumor suppressor(LMW-NTS) on normal human bone marrow CFU-GM and acute myelogenous leukemia cells and its mechanism.
Methods: CFU-GM colony culture, cell culture, Wrigh-Giemsa stain, 3H-TdR incorporation test, agarose gel electrophoresis of DNA fragment and RT-PCR were employed in this research.
Results: The combination of SF and LMW-NTS greatly inhibited the proliferation of acute meylogenous leukemia cells, induced their apoptosis, and down-regulated the c-Myc expression as compared with SF or LMW-NTS alone.
Conclusion: The anti-leukemia effect of the combination of SF and LMW-NTS is superior to SF or LMW-NTS alone.