DNA profiling of azoospermic semen samples from vasectomized males by using Y-PLEX 6 amplification kit

J Forensic Sci. 2003 Jan;48(1):127-9.

Abstract

Post-vasectomized azoospermic semen samples (N = 6) were analyzed for short tandem repeats (STR) on the Y-chromosome by using Y-PLEX 6 and the 310 Genetic Analyzer. We have observed a wide variation in the yield of extracted DNA from 12.5-1,000 ng. This variation was attributed to the number of epithelial and/or white blood cells that are present in these azoospermic samples. DNA profiles of these vasectomized males were obtained for all six Y-STR loci, namely DYS393, DYS 19, DYS389II, DYS390, DYS391, and DYS385 amplified by using the Y-PLEX 6.

Publication types

  • Comparative Study

MeSH terms

  • Alleles
  • Chromosomes, Human, Y
  • DNA / analysis*
  • Female
  • Genotype
  • Humans
  • Male
  • Oligospermia / genetics*
  • Polymerase Chain Reaction / methods*
  • Reagent Kits, Diagnostic*
  • Semen / chemistry
  • Tandem Repeat Sequences
  • Vasectomy*

Substances

  • Reagent Kits, Diagnostic
  • DNA