Chemoenzymatic synthesis of high-mannose type HIV-1 gp120 glycopeptides

Bioorg Med Chem Lett. 2003 Feb 10;13(3):327-30. doi: 10.1016/s0960-894x(02)01025-9.

Abstract

A chemoenzymatic approach to the synthesis of glycoforms of HIV-1 gp120 glycopeptides is described. Thus, the high-mannose type glycopeptides [gp120 (336-342)] containing Man(9), Man(6) and Man(5) moieties, respectively, were synthesized in satisfactory yields via transglycosylation to the acetylglucosaminyl peptide, using the recombinant Arthrobacter Endo-beta-N-acetyl-glucosaminidase (Endo-A) as the key enzyme.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carbohydrate Sequence
  • Chromatography, High Pressure Liquid
  • Glycopeptides / chemical synthesis*
  • Glycopeptides / chemistry
  • HIV Envelope Protein gp120 / chemistry*
  • HIV-1 / chemistry
  • Mannose / chemistry*
  • Molecular Sequence Data
  • Oligosaccharides / chemical synthesis

Substances

  • Glycopeptides
  • HIV Envelope Protein gp120
  • Oligosaccharides
  • Mannose