[Prokaryote expression and western analysis of BcpLH gene of Chinese cabbage]

Shi Yan Sheng Wu Xue Bao. 2000 Dec;33(4):301-7.
[Article in Chinese]

Abstract

BcpLH gene preferentially expressed in folding leaf of Chinese cabbage contains dsRNA-binding domains. The cDNA of BcpLH gene was cloned into a His-fusion expression vector pET-28a (+) and was induced to express in E. coli strain BL21 (DE3). Then, the specific protein was partially purified and the rabbit was immunized to prepare the anti-serum. Meanwhile BcpLH cDNA was cloned into the pMAL-c2 containing the solubizing partner, and then the soluble protein generated. It was demonstrated from Western dot assay that the BcpLH protein was specific. The BcpLH active protein and its anti-serum made it possible to study RNA-binding activity and regulation mechanism in plant development.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Blotting, Western
  • Brassica / genetics*
  • Gene Expression
  • Prokaryotic Cells / metabolism
  • RNA-Binding Proteins / analysis
  • RNA-Binding Proteins / biosynthesis*
  • RNA-Binding Proteins / genetics
  • Recombinant Proteins / analysis
  • Recombinant Proteins / biosynthesis*

Substances

  • RNA-Binding Proteins
  • Recombinant Proteins