[Vascular bundle specific expression of iaaL gene affects the generation frequencies of transgenic tobacco]

Y Xu; W M Ni; Z M Wei; Z H Xu

[Vascular bundle specific expression of iaaL gene affects the generation frequencies of transgenic tobacco]

Shi Yan Sheng Wu Xue Bao. 1999 Mar;32(1):63-71.

[Article in Chinese]

Authors

Y Xu¹, W M Ni, Z M Wei, Z H Xu

Affiliation

¹ National Laboratory of Plant Molecular Genetics, Shanghai Institute of Plant Physiology, Academia Sinica, Shanghai 200032.

PMID: 12548796

Abstract

A vascular bundles specific expressing vector pBAL1 with a promoter AQ630 from rice phenylalanine ammonialyase gene and a gene encoding indoleacetic-lysine synthytase from Pseudomonas syringae subsp. savastanoi was constructed. Affirmed by Southern blotting and RTPCR analysis, the AQ630-iaaL transgenic plants show increasing shoots-regeneration frequency of young stem explants on hormone-free 1/2 MS medium and lower sensibility to IAA when roots were induced from the root explants on the media containing different concentrations of IAA compared to untransformed plants.

Publication types

English Abstract

MeSH terms

Culture Techniques
Gene Expression
Nicotiana / genetics*
Nicotiana / growth & development
Peptide Synthases / genetics*
Plant Roots / growth & development
Plant Stems / growth & development
Plants, Genetically Modified*
Plants, Toxic*

Substances

Peptide Synthases
indoleacetic acid-lysine synthetase