Cloning and expression analysis of rainbow trout Oncorhynchus mykiss interferon regulatory factor 1 and 2 (IRF-1 and IRF-2)

Dev Comp Immunol. 2003 Feb;27(2):111-26. doi: 10.1016/s0145-305x(02)00072-1.

Abstract

Two rainbow trout (Oncorhynchus mykiss) genes for interferon regulatory factors (IRF) 1 and 2 have been cloned and sequenced. The IRF-1 cDNA contains an open reading frame (ORF) of 996 nucleotides that translates into a 331 amino-acid putative peptide, with a 5' untranslated region (UTR) of 145bp and a 3' UTR of 481bp. The IRF-2 cDNA contains a 1035bp ORF that translates into a 344 amino-acid putative peptide, with a 5' UTR of 146bp and a 3' UTR of 925bp. In vivo, IRF-1 and IRF-2 are constitutively expressed in head kidney, gill and spleen but not liver. Both genes were induced in all the tissues examined. IRF-1 but not IRF-2 expression was significantly increased at the site of injection 1 week after DNA vaccination against viral haemorrhagic septicaemia virus. In vitro, IRF-1 and IRF-2 transcripts are present in unstimulated rainbow trout gonad cells and are up-regulated by poly I/C.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cell Line
  • Cloning, Molecular
  • DNA-Binding Proteins / chemistry
  • DNA-Binding Proteins / genetics*
  • Gene Expression
  • Interferon Regulatory Factor-1
  • Interferon Regulatory Factor-2
  • Molecular Sequence Data
  • Novirhabdovirus / immunology
  • Oncorhynchus mykiss / immunology*
  • Phosphoproteins / chemistry
  • Phosphoproteins / genetics*
  • Repressor Proteins*
  • Transcription Factors*
  • Vaccines, DNA / immunology
  • Viral Vaccines / immunology

Substances

  • DNA-Binding Proteins
  • Interferon Regulatory Factor-1
  • Interferon Regulatory Factor-2
  • Phosphoproteins
  • Repressor Proteins
  • Transcription Factors
  • Vaccines, DNA
  • Viral Vaccines