Using meperidine abusers as subjects for this study, we detected in hair meperidine and its metabolites normeperidine, N-OCH3-meperidine and N-AC-meperidine. The content of meperidine and normeperidine of 60 abusers were 103 +/- 130 ng/mg and 117 +/- 143 ng/mg respectively. Meperidine was stably present in hair for at least 20 months. Normeperidine level decreased from hair root to tip. The segmental analysis of hair revealed that there were correlations between the drug abuse history and the distribution of drug along the hair shaft, and between the dose of meperidine and its content presented in hair.