Determination of riboflavin in urine by capillary electrophoresis-blue light emitting diode-induced fluorescence detection combined with a stacking technique

An-Kai Su; Cheng-Huang Lin

doi:10.1016/s1570-0232(02)00849-8

Determination of riboflavin in urine by capillary electrophoresis-blue light emitting diode-induced fluorescence detection combined with a stacking technique

J Chromatogr B Analyt Technol Biomed Life Sci. 2003 Feb 25;785(1):39-46. doi: 10.1016/s1570-0232(02)00849-8.

Authors

An-Kai Su¹, Cheng-Huang Lin

Affiliation

¹ Department of Chemistry, National Taiwan Normal University, 88 Sec. 4, Tingchow Road, Taipei, Taiwan.

PMID: 12535836
DOI: 10.1016/s1570-0232(02)00849-8

Abstract

A simple, inexpensive and reliable method for the simultaneous, routine analysis of riboflavin in urine by capillary electrophoresis-light emitting diode (LED)-induced fluorescence detection is described. Using a blue LED as the light source, the detection limit of riboflavin was determined to be 0.48 microg/ml and was improved to 20 ng/ml when a stacking technique was applied. In the analysis of an actual sample, various concentrations of riboflavin were distributed in the urine samples over a period of 9 h after the ingestion of a vitamin B(2) tablet.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Electrophoresis, Capillary / methods*
Riboflavin / urine*
Sensitivity and Specificity
Spectrometry, Fluorescence

Substances

Riboflavin