The environmental effect of land use becomes one of the most important issues in ecosystem research. This study on phosphorus in runoff was conducted in two contiguous sub-watersheds with three land use types, which located in Meicun watershed of Xuancheng County, Anhui Province. The sub-watersheds differed in land use, but had similar soils and hydrology. The results showed that the runoff dissolved phosphorus (DP) and particulate-associated phosphorus (PAP) concentration of forest-pond-paddy-village system was the highest among the three land use systems. There was no significant difference between DP of the forest-pond system and forest-paddy system. However, the PAP and TP of forest-paddy system was higher than that of forest-pond. The rank of the DP, PAP and TP discharges was wholly same with that of the PAP concentration. About 90% of TP was discharged with the particulate soil. There was a significant linear relationship between the concentration of PAP and the particulate of runoff in three land use systems. But only in the forest-pond-paddy-village system existed a logarithm linear relationship between DP and PAP concentration.