Abstract
Splice variant and authentic mRNAs for procathepsin E were measured at a ratio of 5:1 and 1:2 in Kato 3 and AGS cells, two human gastric adenocarcinoma cell lines. As a result of the precise splicing of the 3'-end of exon 6 to the 5'-end of exon 8, the variant lacked the 142 bp of exon 7 which encodes the second of the Asp residues that operate the catalytic mechanism of aspartic proteinases.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adenocarcinoma / metabolism*
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Alternative Splicing
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Cathepsin E / genetics*
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Cathepsin E / metabolism
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Cathepsins / genetics*
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Cathepsins / metabolism
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Enzyme Precursors / genetics*
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Enzyme Precursors / metabolism
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Exons
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Genetic Variation
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Humans
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Introns
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Polymerase Chain Reaction
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RNA, Messenger / analysis
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RNA, Messenger / isolation & purification
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Stomach Neoplasms / metabolism*
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Tumor Cells, Cultured
Substances
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Enzyme Precursors
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RNA, Messenger
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Cathepsins
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procathepsin E
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Cathepsin E