An alternatively spliced variant of cathepsin E in human gastric adenocarcinoma cells

Biochim Biophys Acta. 2003 Jan 27;1625(2):203-6. doi: 10.1016/s0167-4781(02)00595-x.

Abstract

Splice variant and authentic mRNAs for procathepsin E were measured at a ratio of 5:1 and 1:2 in Kato 3 and AGS cells, two human gastric adenocarcinoma cell lines. As a result of the precise splicing of the 3'-end of exon 6 to the 5'-end of exon 8, the variant lacked the 142 bp of exon 7 which encodes the second of the Asp residues that operate the catalytic mechanism of aspartic proteinases.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenocarcinoma / metabolism*
  • Alternative Splicing
  • Cathepsin E / genetics*
  • Cathepsin E / metabolism
  • Cathepsins / genetics*
  • Cathepsins / metabolism
  • Enzyme Precursors / genetics*
  • Enzyme Precursors / metabolism
  • Exons
  • Genetic Variation
  • Humans
  • Introns
  • Polymerase Chain Reaction
  • RNA, Messenger / analysis
  • RNA, Messenger / isolation & purification
  • Stomach Neoplasms / metabolism*
  • Tumor Cells, Cultured

Substances

  • Enzyme Precursors
  • RNA, Messenger
  • Cathepsins
  • procathepsin E
  • Cathepsin E