Abstract
Based on the topology of the outer membrane protein TolC of Escherichia coli, a new plasmid-encoded system was created which allows the expression of antigenic peptides within permissive, surface-exposed domains of TolC. To assess the capacity of this novel antigen display system, a protective CD4 T-cell epitope of the p60 protein of Listeria monocytogenes was inserted within an extracellular loop of the TolC-protein and expressed in surface-exposed form by attenuated Salmonella enteritidis. Mice immunized orally with this recombinant S. enteritidis live vaccine strain were protected against a lethal challenge with wildtype L. monocytogenes.
MeSH terms
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Administration, Oral
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Animals
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Bacterial Outer Membrane Proteins / genetics*
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Bacterial Proteins / genetics*
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Bacterial Proteins / immunology
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Bacterial Vaccines / immunology*
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Epitopes, B-Lymphocyte / genetics
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Epitopes, B-Lymphocyte / metabolism
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Epitopes, T-Lymphocyte / genetics
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Epitopes, T-Lymphocyte / metabolism
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Escherichia coli
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Escherichia coli Proteins
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Fluorescent Antibody Technique
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Listeria monocytogenes / genetics
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Listeria monocytogenes / immunology
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Listeriosis / immunology
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Listeriosis / prevention & control*
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Membrane Transport Proteins
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Mice
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Mice, Inbred BALB C
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Plasmids
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Recombinant Proteins / genetics
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Recombinant Proteins / metabolism
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Salmonella enteritidis / genetics
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Salmonella enteritidis / metabolism*
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Vaccines, Attenuated / immunology
Substances
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60 kDa protein, Listeria monocytogenes
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Bacterial Outer Membrane Proteins
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Bacterial Proteins
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Bacterial Vaccines
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Epitopes, B-Lymphocyte
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Epitopes, T-Lymphocyte
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Escherichia coli Proteins
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Membrane Transport Proteins
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Recombinant Proteins
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Vaccines, Attenuated
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tolC protein, E coli