Simultaneous determination of oxidative hair dye p-phenylenediamine and its metabolites in human and rabbit biological fluids

Anal Biochem. 2003 Jan 15;312(2):201-7. doi: 10.1016/s0003-2697(02)00501-8.

Abstract

A liquid chromatography with an electrochemical detector method has been developed for the quantitative measurement for three diamine derivatives (p-phenylenediamine, N,N(')-p-phenylenebisacetamide, and 4-aminoacetanilide) in human urine and rabbit blood, urine, and feces. The detection cell consisted of a glassy carbon electrochemical signal obtained with a supporting electrolyte containing 20% methanol-5mM octylammonium orthophosphate (pH 6.30) as the mobile phase. A comparison of the results obtained from HPLC-UV shows agreement.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetanilides / analysis
  • Acetanilides / blood
  • Acetanilides / metabolism
  • Acetanilides / urine
  • Adult
  • Animals
  • Body Fluids / chemistry*
  • Chromatography, High Pressure Liquid
  • Feces / chemistry
  • Female
  • Hair Dyes / analysis*
  • Hair Dyes / metabolism*
  • Humans
  • Male
  • Oxidation-Reduction
  • Phenylenediamines / analysis*
  • Phenylenediamines / blood
  • Phenylenediamines / metabolism*
  • Phenylenediamines / urine
  • Rabbits
  • Sensitivity and Specificity

Substances

  • Acetanilides
  • Hair Dyes
  • Phenylenediamines
  • 4-aminoacetanilide