Abstract
A liquid chromatography with an electrochemical detector method has been developed for the quantitative measurement for three diamine derivatives (p-phenylenediamine, N,N(')-p-phenylenebisacetamide, and 4-aminoacetanilide) in human urine and rabbit blood, urine, and feces. The detection cell consisted of a glassy carbon electrochemical signal obtained with a supporting electrolyte containing 20% methanol-5mM octylammonium orthophosphate (pH 6.30) as the mobile phase. A comparison of the results obtained from HPLC-UV shows agreement.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Acetanilides / analysis
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Acetanilides / blood
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Acetanilides / metabolism
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Acetanilides / urine
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Adult
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Animals
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Body Fluids / chemistry*
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Chromatography, High Pressure Liquid
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Feces / chemistry
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Female
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Hair Dyes / analysis*
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Hair Dyes / metabolism*
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Humans
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Male
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Oxidation-Reduction
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Phenylenediamines / analysis*
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Phenylenediamines / blood
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Phenylenediamines / metabolism*
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Phenylenediamines / urine
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Rabbits
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Sensitivity and Specificity
Substances
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Acetanilides
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Hair Dyes
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Phenylenediamines
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4-aminoacetanilide