Oxysterols induce apoptosis and accumulation of cell cycle at G(2)/M phase in the human monocytic THP-1 cell line

Life Sci. 2003 Feb 7;72(12):1389-99. doi: 10.1016/s0024-3205(02)02377-9.

Abstract

The cytotoxic activity of oxysterols, 7 beta-hydroxycholesterol (7 beta-OHC) and 25-hydroxycholesterol (25-OHC), has been evaluated using various leukemia cell lines. Among the tested cell lines, both oxysterols showed the highest cytotoxicity to THP-1, human monocytic leukemia cell line. These oxysterols induced apoptosis through down-regulation of Bcl-2 expression and activation of caspases. Also, the oxysterols showed the accumulation at G(2)/M phase of cell cycle through down-regulation of cyclin B1 expression. Taken together, these results indicated that both 7 beta-OHC and 25-OHC inhibited the proliferation of THP-1 cells through apoptosis and cell cycle accumulation at G(2)/M phase.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Apoptosis / drug effects*
  • Blotting, Western
  • DNA Fragmentation
  • Flow Cytometry
  • G2 Phase / drug effects*
  • HL-60 Cells / pathology
  • Humans
  • Hydroxycholesterols / pharmacology*
  • Leukemia / pathology
  • Leukemia, Monocytic, Acute / pathology*
  • Leukemia-Lymphoma, Adult T-Cell
  • Mitosis / drug effects*
  • Tumor Cells, Cultured

Substances

  • Hydroxycholesterols
  • cholest-5-en-3 beta,7 alpha-diol
  • 25-hydroxycholesterol