Objective: To establish a HPLC method to determine the contents of nodakenin in Peucedanum.
Method: The determination method was achieved by RP-HPLC on an ODS column by UV detection at 334 nm; with nodakenin as external standard and H2O-CH3OH-CH3CN(75:5:20) as mobile phase.
Result: Several samples of 6 species of Peucedanum were analyzed by this method. The contents of nodakenin ranged from 0.005%-3.090%.
Conclusion: Nodakenin cannot be used to control the quality of Peucedanum for pharmaceutical use, but this method is a simple and feasible way to control the quality of P. decursivum.