Objective: To study the hairy root induction and hormone-free in vitro liquid cultivation of Pueraria lobata (Willd) Ohwi.
Method: Co-cultivation of super-virulent Agrobacterium rhizogenes R1601 with P. lobata leaves in vitro.
Results: Hairy roots of rapid growth, high branches and plagiotropism developed vigorously on the surface of leaves, exhibiting rapid growth and resistance to kanamycin in hormone-free medium in vitro.
Conclusion: A method of hairy root induction with A. rhizogenes as well as a system hairy root liquid cultivation in vitro for P. lobata have been established.