Objective: Establishment of a gene expression profile associated with differentiation inducing the glioma cells was made possible.
Method: The expression level of 18 000 genes in glioma cells was evaluated before and after induction with sodium phenyl-butyrate for 2 hours or 6 days by cDNA array technique, with the results proved by multi-dot blot.
Results: Ninety-eight gene expressions in the glioma cells were changed after the induction, with some genes in transcription and translation systems down-regulated, some oncogenes down-regulated, and some differentiation or apoptosis genes up-regulated. Eighteen unknown EST fragments were changed also.
Conclusion: A gene expression profile associated with differentiation-inducing the glioma cells including 98 genes has been established.