[Detection of rotavirus RNA using DIG labelled probe prepared by polymerase chain reaction]

Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 1998 Mar;12(1):77-9.
[Article in Chinese]

Abstract

The digoxigenin (DIG) labelled cDNA probe of rotavirus was directly prepared by polymerase chain reaction (PCR). The results of cDNA-RNA hybridization showed that the DIG-cDNA probe exhibits rotavirus specificity and can detect as tiny as 10 pg rotavirus RNA. 120 fecal samples of diarrhea from infants and young children were tested by dot-blot hybridization. It was shown that the positive rate of dot-blot hybridization(65.0%) was significantly higher than that of PAGE(49.1%). This study indicated that direct preparation of DIG-labelled rotavirus-cDNA probe by PCR is much faster and simpler than common method of labeling.

Publication types

  • Comparative Study
  • English Abstract

MeSH terms

  • Adult
  • DNA, Complementary
  • Digoxigenin
  • Electrophoresis, Polyacrylamide Gel
  • Humans
  • Infant
  • Polymerase Chain Reaction
  • RNA, Viral / analysis*
  • Rotavirus / isolation & purification*
  • Rotavirus Infections / virology*

Substances

  • DNA, Complementary
  • RNA, Viral
  • Digoxigenin