[Studies on separation and identification of Rhizoma coptidis brugs based on microemulsion thin-layer chromatography]

Zhongguo Zhong Yao Za Zhi. 2000 May;25(5):262-5.
[Article in Chinese]

Abstract

Objective: To investigate the use of microemulsion TLC for the separation and identification of Rhizoma Coptidis drugs.

Method: Thirteen Rhizoma Coptidis drugs were separated and identified with six kinds of SDS/n-C4H9OH/n-C7H16/H2O microemulsions as mobile phase on polyamide film. Effects of microemulsions on separation were investigated.

Results: The best mobile phase was the O/W microemulsion(containing 75% water). Compared with general mobile phases, the separation and detection could be improved and the test conditions were more tolerant.

Conclusion: This new method is simple, accurate and efficient for the separation and identification of Rhizoma Coptidis drugs.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Berberine / analysis
  • Berberine Alkaloids / analysis
  • Chromatography, Thin Layer / methods
  • Coptis chinensis
  • Drug Combinations
  • Drugs, Chinese Herbal / analysis*
  • Drugs, Chinese Herbal / chemistry*
  • Plants, Medicinal / chemistry*
  • Plants, Medicinal / classification
  • Ranunculaceae / chemistry*
  • Ranunculaceae / classification

Substances

  • Berberine Alkaloids
  • Drug Combinations
  • Drugs, Chinese Herbal
  • Berberine
  • Coptidis rhizoma extract
  • palmatine