Abstract
There are several kinases in Saccharomyces cerevisiae that phosphorylate the CTD of RNA polymerase II, but specific and distinct functions of the phospho-CTDs generated by the different kinases are not well understood. A genetic screen for suppressors of loss of yeast CTD kinase I (CTDK-I) function (by deletion of the catalytic subunit gene CTK1) identified PTI1, a potential 3' cleavage/polyadenylation factor. Genetic and physical interactions connect Pti1p to components of CF IA and CF II/CPF, and mutations of PTI1 or CTK1 affect 3' cleavage site choice and transcript abundance of particular genes. Therefore, one important function of the CTDK-I-generated phospho-CTD appears to be the coupling of transcription to 3' processing of pre-mRNAs by a Pti1p-containing complex.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Genes, Essential
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Genes, Fungal
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Molecular Sequence Data
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Peptide Fragments / chemistry
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Poly A / metabolism
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Protein Kinases / metabolism*
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Protein Serine-Threonine Kinases / metabolism*
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Protein Subunits / chemistry
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Protein Subunits / genetics
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Protein Subunits / metabolism
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RNA Polymerase II / chemistry
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RNA Polymerase II / genetics*
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RNA Polymerase II / metabolism*
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RNA Precursors / metabolism*
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Saccharomyces cerevisiae / enzymology*
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Saccharomyces cerevisiae / genetics
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Saccharomyces cerevisiae / growth & development
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Saccharomyces cerevisiae Proteins*
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Sequence Alignment
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Sequence Homology, Amino Acid
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Substrate Specificity
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Transcription, Genetic
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mRNA Cleavage and Polyadenylation Factors
Substances
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PTI1 protein, S cerevisiae
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Peptide Fragments
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Protein Subunits
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RNA Precursors
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Saccharomyces cerevisiae Proteins
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mRNA Cleavage and Polyadenylation Factors
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Poly A
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Protein Kinases
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carboxy-terminal domain kinase
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Protein Serine-Threonine Kinases
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RNA Polymerase II