Development of a dual recombinant vaccine to protect small ruminants against peste-des-petits-ruminants virus and capripoxvirus infections

G Berhe; C Minet; C Le Goff; T Barrett; A Ngangnou; C Grillet; G Libeau; M Fleming; D N Black; A Diallo

doi:10.1128/jvi.77.2.1571-1577.2003

Development of a dual recombinant vaccine to protect small ruminants against peste-des-petits-ruminants virus and capripoxvirus infections

J Virol. 2003 Jan;77(2):1571-7. doi: 10.1128/jvi.77.2.1571-1577.2003.

Authors

G Berhe¹, C Minet, C Le Goff, T Barrett, A Ngangnou, C Grillet, G Libeau, M Fleming, D N Black, A Diallo

Affiliation

¹ Cirad, Programme Santé Animale, Campus International de Baillarguet, 34398 Montpellier Cedex 05, France.

Abstract

A recombinant capripoxvirus vaccine containing a cDNA of the peste-des-petits-ruminants virus (PPRV) fusion protein gene was constructed. A quick and efficient method was used to select a highly purified recombinant virus clone. A trial showed that a dose of this recombinant as low as 0.1 PFU protected goats against challenge with a virulent PPRV strain.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animal Diseases / prevention & control*
Animals
Base Sequence
Capripoxvirus / immunology
Capripoxvirus / isolation & purification*
DNA Primers
DNA, Complementary
Dose-Response Relationship, Immunologic
Peste-des-Petits-Ruminants / prevention & control*
Peste-des-Petits-Ruminants / veterinary
Peste-des-petits-ruminants virus / immunology
Poxviridae Infections / prevention & control*
Poxviridae Infections / veterinary
Poxviridae Infections / virology
Ruminants
Viral Vaccines / administration & dosage
Viral Vaccines / immunology*

Substances

DNA Primers
DNA, Complementary
Viral Vaccines