Abstract
Radixin is a member of the ERM proteins, which cross-link plasma membranes and actin filaments. The N-terminal FERM domains of ERM proteins interact with Na(+)/H(+)-exchanger regulatory factors (NHERFs), which are PDZ-containing adaptor proteins, to modulate the ion-channel activity. Here, crystals of complexes between the radixin FERM domain and the C-terminal regions of NHERF and NHERF2 have been obtained. The crystals of the FERM-NHERF complex were found to belong to space group P2(1)2(1)2(1), with unit-cell parameters a = 69.38 (2), b = 146.27 (4), c = 177.76 (7) A. The crystal contains four complexes in the asymmetric unit. An intensity data set was collected to a resolution of 2.50 A.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Blood Proteins / chemistry*
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Blood Proteins / metabolism
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Crystallization
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Crystallography, X-Ray
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Cytoskeletal Proteins / chemistry*
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Cytoskeletal Proteins / metabolism
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Humans
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Membrane Proteins / chemistry*
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Membrane Proteins / metabolism
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Mice
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Molecular Sequence Data
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Phosphoproteins / chemistry*
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Phosphoproteins / metabolism
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Protein Structure, Tertiary
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Recombinant Fusion Proteins / chemistry
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Recombinant Fusion Proteins / metabolism
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Sodium-Hydrogen Exchangers
Substances
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Blood Proteins
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Cytoskeletal Proteins
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Membrane Proteins
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Phosphoproteins
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Recombinant Fusion Proteins
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Sodium-Hydrogen Exchangers
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sodium-hydrogen exchanger regulatory factor
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radixin