Objective: To observe the effect of cytochalasin B on the denucleation of K562 cells.
Methods: K562 cells were grown in RPMI 1640 medium supplemented with 10% calf serum and 4 microL cytochalasin B (CB). Denucleation was induced in the cultured cells by CB, and the cells were examined by phase contrast microscopy and Giemsa staining respectively.
Results: The denucleation of K562 cells induced by CB was clearly observed, and the cell proliferation was obviously inhibited.
Conclusion: The denucleation efficiency of K562 cells is positively correlated with CB concentrations and the duration of CB treatment. CB at low doses may inhibit the cell proliferation and at high doses causes cell death.