Proteolysis during the isoelectric focusing step of two-dimensional gel electrophoresis may be a common problem

Anal Biochem. 2002 Dec 15;311(2):182-6. doi: 10.1016/s0003-2697(02)00389-5.
No abstract available

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Electrophoresis, Gel, Two-Dimensional / standards
  • Endopeptidases / metabolism*
  • Hordeum / enzymology
  • Isoelectric Focusing / standards*
  • Peptide Fragments
  • Protease Inhibitors
  • Proteins / analysis
  • Proteins / isolation & purification
  • Proteomics / methods*
  • Proteomics / standards
  • beta-Amylase / isolation & purification
  • beta-Amylase / metabolism

Substances

  • Peptide Fragments
  • Protease Inhibitors
  • Proteins
  • beta-Amylase
  • Endopeptidases