Probing the structural dynamics of nucleic acids by quantitative time-resolved and equilibrium hydroxyl radical "footprinting"

Michael Brenowitz; Mark R Chance; Gauri Dhavan; Keiji Takamoto

doi:10.1016/s0959-440x(02)00366-4

Probing the structural dynamics of nucleic acids by quantitative time-resolved and equilibrium hydroxyl radical "footprinting"

Curr Opin Struct Biol. 2002 Oct;12(5):648-53. doi: 10.1016/s0959-440x(02)00366-4.

Authors

Michael Brenowitz¹, Mark R Chance, Gauri Dhavan, Keiji Takamoto

Affiliation

¹ Department of Biochemistry, Center for Synchrotron Biosciences, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA. brenowit@aecom.yu.edu

PMID: 12464318
DOI: 10.1016/s0959-440x(02)00366-4

Abstract

For many years, hydroxyl radical footprinting has been an insightful probe of the solvent accessibility of local regions of nucleic acid structure. Recently, quantitative applications of this technique have been developed that allow time-resolved and equilibrium analysis of transitions involving nucleic acid ligand binding and conformation change to be analyzed incisively.

Publication types

Review

MeSH terms

DNA / chemistry*
DNA Footprinting / methods*
Hydroxyl Radical
Nucleic Acid Conformation
Nucleic Acids / chemistry*
RNA / chemistry*

Substances

Nucleic Acids
Hydroxyl Radical
RNA
DNA