Abstract
It is 20 years since site-directed mutagenesis was first used to modify the active site of an enzyme of known structure and mechanism. Since then, this method has contributed far-reaching insights into catalysis, specificity, stability and folding of proteins. Engineered proteins are now being used in industry and for the improved treatment of human disease.
Publication types
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Research Support, Non-U.S. Gov't
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Review
MeSH terms
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Antibodies / genetics
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Antibodies / metabolism
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Binding Sites
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Hemoglobins / genetics
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Hemoglobins / metabolism
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Humans
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Insulin / chemistry
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Insulin / genetics
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Molecular Structure
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Mutagenesis, Site-Directed
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Protein Conformation*
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Protein Engineering*
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Tyrosine-tRNA Ligase / chemistry
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Tyrosine-tRNA Ligase / genetics
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Tyrosine-tRNA Ligase / metabolism*
Substances
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Antibodies
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Hemoglobins
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Insulin
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Tyrosine-tRNA Ligase