Protein engineering 20 years on

Nat Rev Mol Cell Biol. 2002 Dec;3(12):964-70. doi: 10.1038/nrm975.

Abstract

It is 20 years since site-directed mutagenesis was first used to modify the active site of an enzyme of known structure and mechanism. Since then, this method has contributed far-reaching insights into catalysis, specificity, stability and folding of proteins. Engineered proteins are now being used in industry and for the improved treatment of human disease.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Antibodies / genetics
  • Antibodies / metabolism
  • Binding Sites
  • Hemoglobins / genetics
  • Hemoglobins / metabolism
  • Humans
  • Insulin / chemistry
  • Insulin / genetics
  • Molecular Structure
  • Mutagenesis, Site-Directed
  • Protein Conformation*
  • Protein Engineering*
  • Tyrosine-tRNA Ligase / chemistry
  • Tyrosine-tRNA Ligase / genetics
  • Tyrosine-tRNA Ligase / metabolism*

Substances

  • Antibodies
  • Hemoglobins
  • Insulin
  • Tyrosine-tRNA Ligase